Results
Phase I.
The research explores the use of Spirulina-derived complex extracts—rich in both sulfated polysaccharides and proteins—as reducing and stabilizing agents for the green synthesis of Ag NPs. Among three experimental conditions tested, the extract from biomass grown under a light/dark cycle with zinc acetate supplementation yielded the highest extract quantity and was selected for further work. Ag NPs synthesized using this extract were characterized by TEM and FTIR, confirming their nanoscale structure and effective integration with biomolecules. These Ag NPs were subsequently biofunctionalized by combining with Spirulina-derived protein extracts to enhance biocompatibility. A chemical synthesis route was also used to prepare Ag NPs functionalized with ibuprofen. Structural investigations were conducted using advanced microscopy techniques. The project achieved all its deliverables and objectives, including the development of functional nanosystems and dissemination of results via international conferences and publications.